ABSTRACT
Objective To analyze the problems and reasons in the clinical medicine postgraduate cultivation.We stated the competency of clinical medicine postgraduates according to the transition of health demands global medical development and practical requirements for clinical personnel. Me thods Questionnaire surveys combined with interviews were conducted, and statistical analysis was performed. In the first part, we selected the permanent staff with qualifications of medical clinicians, including: chief physicians, associate chief physicians, physicians, residents, accounting for 72％ of the interviewees; And related personnel, including nurses, administrators and patients, were also interviewed, accounting for 28％ of the total. 90 questionnaires were distributed, and 86 valid questionnaires were recovered, with an effective rate of 95.56％. In the second part, graduate students with clinical professional degrees were selected for interviews, 96 questionnaires were distributed, and 92 valid questionnaires were collected, and the effective rate was 95.83％.Re s ults There was a certain gap between the expected goal and the actual situation of the post competency of clinical professional degree graduate students, and the gap had a certain statistical significance. The clinical professional degree graduate students paid more attention to the doctor-patient communication, teamwork. Conclus ions We elaborated the required post competence for clinical medicine postgraduates on the basis of its content and characteristic. We propose to strengthen the cultivation of clinical medicine postgraduates by the reform and support of the government enhanced construction of teachers improved course plan and cultivation process perfected assessment system in order to make the students competent to their posts.
ABSTRACT
Objective: To evaluate the role of decellularized vascular scaffolds coated with anti-CD34 antibodies in capturing endothelial progenitor cells from the circulation to participate in recellularization. Methods: Fresh caprine forelimb arteries were treated with repeated frozen/thawing, ultrahigh pressure and SDS to prepare decellularized vascular scaffolds. After reaction with photochemical crosslinker SANPAH, anti-rabbit CD34 was coated onto the decellularized vascular scaffolds using ultraviolet ray. Twenty New Zealand white rabbits were performed for right lower-abdominal pedicled skin flap, which were supplied by branches of femoral artery. Whole defects (1 cm) were made in femoral arteries and end-to-end anastomosis repaired by 10 cross-linked and 10 non-cross-linked scaffolds in experimental group and control group, respectively. The patency of the pedicle was observed through color and appearance of the flap postoperatively. After transplantation, patency rate and cell seeding were detected by Doppler, DSA, and pathological test. Results:. Flaps of the experimental group had good blood supply after transplantation, while swelling and necrosis could be found in the control group. Doppler and DSA showed that the 7 of 10 cross-linked scaffolds remained patent for 4 weeks and there was no stenonsis, but all the scaffolds were obstructed in the control group after 1 week. Hematoxylin and eosin staining revealed that the inner layers of cross-linked scaffolds were partly covered with endothelial cells four weeks later, and there was no noticeable stenosis. In contrast, thrombosis formation was noticed in the control group and there was no cell coverage one week after operation. Conclusion: Cross-linked scaffolds with anti-CD34 antibodies are superior to bare scaffolds in early postoperative anticoagulation and patency.